Chefs de Project

Camille LOCHT DRCE Inserm

Françoise JACOB-DUBUISSON DR1 CNRS

Alain BAULARD DR2 Inserm

Nathalie MIELCAREK DR2 Inserm

Philip SUPPLY DR1 CNRS

Chercheurs

Rudy ANTOINE, CRCN Inserm

Nicolas BLONDIAUX, University Hospital Practitioner

Stéphane CAUCHI, CRCN CNRS

Loïc COUTTE CRCN, Inserm

Christine DEMANCHE, Associate Professor Univ. Lille2

Olivier GAILLOT, MCU-PH Univ. Lille2

Carine ROUANET, CR Institut Pasteur de Lille

Romain VEYRON-CHURLET, CRCN CNRS

Ingénieurs et techniciens

Anne-Sophie DEBRIE IR Institut Pasteur de Lille

Jonathan CHATAGNON, IE Institut Pasteur de Lille

Sophie LECHER TCS Institut Pasteur de Lille

Emmanuelle PETIT TCS Inserm

Dominique RAZE IR Inserm

Stéphanie VERPOORT TCS Institut Pasteur de Lille

Post-doctorants et doctorants

Thomas BELCHER, Post-Doc

Kamel DJAOUT, Post-Doc

Violaine DUBOIS, Post-Doc

Rosangela-Maria FRITA, Bioversys

Fethi KHITER, PhD Student

Alex RIVERA-MILLOT, PhD Student

Gauthier ROY, PhD Student

Anaïs Thiriard, Post-Doc


Actuels PhD - Post-Docs

Fethi Khiter (2019-2022), Université de Lille
Fellowship: Agence Régionale de Santé
Title : Role of myeloid cells of the respiratory tract in immunity induced by the nasal attenuated vaccine BPZE1 against whooping cough
Supervisor: N. Mielcarek

 

Alex Rivera (2016-2019), Université de Lille
Fellowship:    University of Lille/Region Hauts de France
Title:    Copper homeostasis in Bordetella pertussis and its relationship with virulence
Supervisor:    F. Jacob-Dubuisson

Gauthier Roy (2018-2021)  Université de Lille
Fellowship:    Université de Lille
Title: Characterization of a new copper transport system in B; pertussis: mode of action and regulation
Supervisor: F. Jacob-Dubuisson

Thomas Belcher (UK), since 2017
Financial supports: CPER CTRL IPL

Kamel Djaout (Algeria), 2016 - ongoing
Financial supports: Industrial partnership (Bioversys-GSK)
Publications:
-    Blondiaux N, Moune M, (…), Djaout K, (…), Baulard AR (2017) Reversion of antibiotic resistance in Mycobacterium tuberculosis by spiroisoxazoline SMARt-420. Science 355:1206-1211.

Rosangela-Maria Frita (Portugal), 2015 - ongoing
Financial supports: Bioversys
Publications:
-    Rens C, Laval F, Daffé M, Denis O, Frita R, Baulard A, Wattiez R, Lefèvre P, Fontaine V (2016) Effects of Lipid-Lowering Drugs on Vancomycin Susceptibility of Mycobacteria. Antimicrob Agents Chemother 60:6193-9.
-    Blondiaux N, Moune M, Desroses M, Frita R, (…), Djaout K, (…), Baulard AR (2017) Reversion of antibiotic resistance in Mycobacterium tuberculosis by spiroisoxazoline SMARt-420. Science 355:1206-1211.
-    Tatum NJ, Liebeschuetz JW, Cole JC, Frita R, Herledan A, Baulard AR, Willand N, Pohl E (2017) New active leads for tuberculosis booster drugs by structure-based drug discovery. Org Biomol Chem 15:10245-10255.

 

Anciens doctorants & Postdocs

Salam Al Ashkar (2016-2019), Université de Lille II
Fellowship:  Association AZM and Saadeh
Title: Prevalence of drug-resistant tuberculosis assessed by next-generation sequencing: an 18-month nationwide study in Lebanon
Supervisor:  P. Supply
Current employment:

 

Elian Dupré (2009-2013), Université de Lille II
Fellowship:  French Research Ministry for 3 years and FRM for the 4th
Title: Virulence regulation in Bordetella pertussis : BvgS, an original model for a two-component system receptor
Supervisor:  F. Jacob-Dubuisson
Current employment: post-doctoral fellow, University of Lille 1
Jérémy Guérin (2011-2014), Université de Lille II
Fellowship:  Institut Pasteur of Lille/ Région Nord-Pas de Calais.
Title: Conformationnal dynamics of FhaC, the transporter across the outer membrane of the filamentous hemaggutinin in Bordetella pertussis
Supervisor:  F. Jacob-Dubuisson
Current employment: post-doctoral fellow, NIH, USA
Elodie Lesne (2013-2016), Université de Lille II
Fellowship:  INSERM/ Région Nord-Pas de Calais.
Title:  Virulence regulation by the whooping cough agent Bordetella pertussis : signaling by the sensor-kinase BvgS
Supervisor:  F. Jacob-Dubuisson
Current employment: scientist at Public Health England, Porton Down, Salisbury, UK
Marion Prieri (2015-2018), Université de Lille
Fellowship:    Institut Pasteur de Lille/ Région Haut de France
Title: Reprogramming the anti-TB pro-drugs: design, development and biological evaluation of inhibitors of transcriptional regulators
Co-SupervisorS:    Alain Baulard / Nicolas Willand
Current employment: Newcastle University, Newcastle-upon-Tyne, UK

Anaïs Thiriard (2016-2019), Université de Lille
Fellowship:    Institut Pasteur de Lille
Title : Study on the functionality of anti-pertussis antibodies
Supervisor: Camille Locht
Current employment: post-doctoral fellow, University of Lille 1

Catherine Baud (France), from 2010 to 2014
Financial support: ANR grant DYN FHAC
Publications:
-    Baud C, Guérin J, Petit E, Lesne E, Dupré E, Locht C, Jacob-Dubuisson F (2014) Translocation path of a substrate protein through its Omp85 transporter. Nat Commun 5: 5271.
-    Guérin J, Baud C, Touati N, Saint N, Willery E, Locht C, Vezin H, Jacob-Dubuisson F (2014) Conformational dynamics of protein transporter FhaC: large-scale motions of plug helix. Mol Microbiol 92: 1164-1176.

Elian Dupré (France), from 2013 to 2016
Financial support: ANR grant MECA VENUS
Publications:
-    Dupre E, Herrou J, Lensink MF, Wintjens R, Vagin A, Lebedev A, Crosson S, Villeret V, Locht C, Antoine R, Jacob-Dubuisson F (2015) Virulence regulation with Venus flytrap domains: structure and function of the periplasmic moiety of the sensor-kinase BvgS. PLoS Pathog 11: e1004700.
-    Dupre E, Lesne E, Guerin J, Lensink MF, Verger A, de Ruyck J, Brysbaert G, Vezin H, Locht C, Antoine R, Jacob-Dubuisson F (2015) Signal transduction by BvgS sensor kinase. Binding of modulator nicotinate affects the conformation and dynamics of the entire periplasmic moiety. J Biol Chem 290: 26473.

Jeremy Guerin (France), 2015
Financial support: ANR grant DYN FHAC
Publication:
-    Guerin J, Saint N, Baud C, Meli AC, Etienne E, Locht C, Vezin H, Jacob-Dubuisson F (2015) Dynamic interplay of membrane-proximal POTRA domain and conserved loop L6 in Omp85 transporter FhaC.  Mol Microbiol 98: 490-501.

Hannah Kammoun (Tunisia), oct. 2012 to Dec. 2013
Financial support: FP7 NewTBVac
Publication:
-    Feunou PF, Kammoun H, Debrie AS, Locht C (2014) Heterologous prime-boost immunization with live attenuated B. pertussis BPZE1 followed by acellular pertussis vaccine in mice. Vaccine 32:4281428-8.

Raphaella Iantomasi 2015-2016
Financial support: Lille European Metropolitan (MEL)
Publication:
-    Iantomasi et al (in preparation) Low virulence of Mycobacterium canettii strains in cellular infection models

Martin Moune (Cameroon/France), from 2014 to 2017;
Financial supports: PRIM (2014-2016) and GSK-Bioversys industrial partnership (2016-2018)
Publications:
-    Blondiaux N, Moune M*, (…), Baulard AR (2017) Reversion of antibiotic resistance in Mycobacterium tuberculosis by spiroisoxazoline SMARt-420. Science 355:1206-1211. *Co-first author
-    Wohlkönig A, Remaut H, Moune M, Tanina A, Meyer F, Desroses M, Steyaert J, Willand N, Baulard AR, Wintjens R (2017) Structural analysis of the interaction between spiroisoxazoline SMARt-420 and the Mycobacterium tuberculosis repressor EthR2. Biochem Biophys Res Commun 487:403-408.

Luis Solans (Spain), from 2015 to January 31st, 2018
Financial supports: ILIAD Biotechnologies (industrial support)
Publications:
-    Rivera-Millot A, Lesne E, Solans L, Coutte L, Bertrand-Michel J, Froguel P, Dhennin V, Hot D, Locht C, Antoine R, Jacob-Dubuisson F (2017) Characterization of a Bvg-regulated fatty acid methyl-transferase in Bordetella pertussis. PLoS One 12:e0176396
-    Hovingh ES, Mariman R, Solans L, Hijdra D, Hamstra HJ, Jongerius I, van Gent M, Mooi F, Locht C, Pinelli E (2018) Bordetella pertussis pertactin knock-out strains reveal immunomodulatory properties of this virulence factor. Emerg Microbes Infect 7:39.