Leveraging Sortase A Electrostatics for Powerful Transpeptidation Reactions
05/21/2025
Transpeptidase enzymes are versatile and highly selective tools for peptide and protein engineering. Among them, the sortase A pentamutant (SrtA-5M) has been widely employed for site-specific protein and peptide modification, including cyclization, immobilization, and conjugation with drug payloads.
In this study, we harness the electrostatic properties of SrtA-5M to enhance its transpeptidation efficiency by incorporating short, charged peptide modules into the substrates. This strategy results in a highly efficient and user-friendly reaction, compatible with recombinant protein expression and enabling exceptionally clean protein labeling and protein–protein conjugation.
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