Research Articles


1.      Amman F, D'Halluin A, Antoine R, Huot L, Bibova I, Keidel K, Slupek S, Bouquet P, Coutte L, Caboche S, Locht C, Vecerek B, Hot D (2018) Primary transcriptome analysis reveals importance of IS elements for the shaping of the transcriptional landscape of Bordetella pertussis. RNA Biol 23:1-35.

2.      Barbier M, Dumitrescu O, Pichat C, Carret G, Ronnaux-Baron AS, Blasquez G, Godin-Benhaim C, Boisset S, Carricajo A, Jacomo V, Fredenucci I, Pérouse de Montclos M, Genestet C, Flandrois JP, Ader F, Supply P, Lina G, Wirth T, Rasigade JP (2018) Changing patterns of human migrations shaped the global population structure of Mycobacterium tuberculosis in France. Sci Rep 8:5855.

3.      CRyPTIC Consortium and the 100,000 Genomes Project, (…), Supply P [as chairman of steering committee of the Cryptic consortium], (…), Zhu B (2018) Prediction of Susceptibility to First-Line Tuberculosis Drugs by DNA Sequencing. N Engl J Med 379:1403-1415. 

4.      Debrie AS, Coutte L, Raze D, Mooi F, Alexander F, Gorringe A, Mielcarek N, Locht C (2018) Construction and evaluation of Bordetella pertussis live attenuated vaccine strain BPZE1 producing Fim3. Vaccine 36:1345-1352. 

5.      Heinrichs A, Argudín MA, De Mendonça R, Deplano A, Roisin S, Dodémont M, Coussement J, Filippin L, Dombrecht J, De Bruyne K, Huang TD, Supply P, Byl B, Glupczynski Y, Denis O (2018) An outpatient clinic as a potential site of transmission for an outbreak of NDM-producing Klebsiella pneumoniae ST716: a study using whole-genome sequencing. Clin Infect Dis. July 18. [Epub ahead of print].

6.      Hovingh ES, Mariman R, Solans L, Hijdra D, Hamstra HJ, Jongerius I, van Gent M, Mooi F, Locht C, Pinelli E (2018) Bordetella pertussis pertactin knock-out strains reveal immunomodulatory properties of this virulence factor. Emerg Microbes Infect 7:39.

7.      Jajou R, Kamst M, van Hunen R, de Zwaan CC, Mulder A, Supply P, Anthony R, van der Hoek W, van Soolingen D (2018) Occurrence and Nature of Double Alleles in Variable-Number Tandem-Repeat Patterns of More than 8,000 Mycobacterium tuberculosis Complex Isolates in The Netherlands. J Clin Microbiol 56: e00761-17.

8.      Kowalewicz-Kulbat M, Szpakowski P, Locht C, Biet F, Kaplonek P, Krawczyk KT, Pestel J, Rudnicka W (2018) Tuberculin skin test reaction is related to memory, but not naive CD4+ T cell responses to mycobacterial stimuli in BCG-vaccinated young adults. Vaccine 36:4566-4577.

9.      Lesne E, Dupré E, Lensink MF, Locht C, Antoine R, Jacob-Dubuisson F (2018) Coiled-Coil Antagonism Regulates Activity of Venus Flytrap-Domain-Containing Sensor Kinases of the BvgS Family. MBio 9: e02052-17.

10.    Lesne E, Coutte L, Solans L, Slupek S, Debrie AS, Dhennin V, Froguel P, Hot D, Locht C, Antoine R, Jacob-Dubuisson F (2018) Distinct virulence range for infection of mice by Bordetella pertussis revealed by engineering of the sensor-kinase BvgS. PloS One 13:e0204861.

11.    Makhado NA, Matabane E; Faccin, M; Pinçon, C; Jouet, A; Boutachkourt, F; Goeminne, L; Gaudin, C; Maphalala G, Beckert P, Niemann S, Delvenne JC, Delmée M, Razwiedani L, Nchabeleng M, Supply P*, De Jong BC*, André E* (2018) *Co-senior authors. Outbreak of multidrug-resistant tuberculosis in South Africa undetected by WHO-endorsed commercial tests. An observational study. Lancet Inf Dis. Oct 17. [Epub ahead of print].

12.    Naninck T, Coutte L, Mayet C, Contreras V, Locht C, Le Grand R, Chapon C (2018) In vivo imaging of bacterial colonization of the lower respiratory tract in a baboon model of Bordetella pertussis infection and transmission. Sci Rep 8:12297

13.    Pedersen MK, Andersen AB, Folkvardsen DB, Rasmussen EM, Svensson E, Lillebaek L, Supply P (2018) Set-up and validation of Mycobacterial Interspersed Repetitive Unit-Variable Number of Tandem Repeat (MIRU-VNTR) analysis of Mycobacterium tuberculosis using BioNumerics® software. PLoS One. In press.

14.    Prieri M, Frita R, Probst N, Sournia-Saquet A, Bourotte M, Déprez B, Baulard AR, Willand N (2018) Efficient analoging around ethionamide to explore thioamides bioactivation pathways triggered by boosters in Mycobacterium tuberculosis. Eur J Med Chem 159:35-46. 

15.    Raze D, Verwaerde C, Deloison G, Werkmeister E, Coupin B, Loyens M, Brodin P, Rouanet C, Locht C (2018) Heparin-Binding Hemagglutinin Adhesin (HBHA) Is Involved in Intracytosolic Lipid Inclusions Formation in Mycobacteria. Front Microbiol 9:2258.

16.    Solans L, Debrie AS, Borkner L, Aguiló N, Thiriard A, Coutte L, Uranga S, Trottein F, Martín C, Mills KHG, Locht C (2018) IL-17-dependent SIgA-mediated protection against nasal Bordetella pertussis infection by live attenuated BPZE1 vaccine. Mucosal Immunol. August 16. [Epub ahead of print].

17.    Song OR, Queval CJ, Iantomasi R, Delorme V, Marion S, Veyron-Churlet R, Werkmeister E, Popoff M, Ricard I, Jouny S, Deboosere N, Lafont F, Baulard A, Yeramian E, Marsollier L, Hoffmann E, Brodin P (2018) ArfGAP1 restricts Mycobacterium tuberculosis entry by controlling the actin cytoskeleton. EMBO Rep 19:29-42

18.    Veyron-Churlet R, Dupres V, Saliou JM, Lafont F, Raze D, Locht C (2018) Rv0613c/MSMEG_1285 interacts with HBHA and mediates its proper cell-surface exposure in mycobacteria. Int J Mol Sci 19.

19.    Wanlapakorn N, Maertens K, Chaithongwongwatthana S, Srimuan D, Suratannon N, Vongpunsawad S, Mai Phuong Tran T, Hens N, Van Damme P, Locht C, Poovorawan Y, Leuridan E (2018) Assessing the reactogenicity of Tdap vaccine administered during pregnancy and antibodies to Bordetella pertussis antigens in maternal and cord sera of Thai women. Vaccine 36:1453-1459. 




1.     Jacob-Dubuisson F, Mechaly A, Betton J-M, Antoine R (2018) Structural insights into the signaling mechanisms of two-component systems. Nature Rev Microbiol 16:585-593.

2.     Thiriard A, Raze D, Locht C (2018) Diversion of complement-mediated killing by Bordetella. Microbes Infect. Feb 14. [Epub ahead of print].




Research Articles


1.     Blondiaux N, Moune M, Desroses M, Frita R, Flipo M, Mathys V, Soetaert K, Kiass M, Delorme V, Djaout K, Trebosc V, Kemmer C, Wintjens R, Wohlkönig A, Antoine R, Huot L, Hot D, Coscolla M, Feldmann J, Gagneux S, Locht C, Brodin P, Gitzinger M, Déprez B, Willand N, Baulard AR (2017) Reversion of antibiotic resistance in Mycobacterium tuberculosisby spiroisoxazoline SMARt-420. Science 355:1206-1211.

2.     Costa-Gouveia J, Pancani E, Jouny S, Machelart A, Delorme V, Salzano G, Iantomasi R, Piveteau C, Queval CJ, Song OR, Flipo M, Deprez B, Saint-André JP, Hureaux J, Majlessi L, Willand N, Baulard A, Brodin P, Gref R (2017) Combination therapy for tuberculosis treatment: pulmonary administration of ethionamide and booster co-loaded nanoparticles. Sci Rep 7:5390.

3.     Deboosère N, Iantomasi R, Queval CJ, Song OR, Deloison G, Jouny S, Debrie AS, Chamaillard M, Nigou J, Cohen-Gonsaud M, Locht C, Brodin P, Veyron-Churlet R (2017) LppM impact on the colonization of macrophages by Mycobacterium tuberculosis. Cell Microbiol 19: e12619

4.     Dreesman A, Corbière V, Dirix V, Smits K, Debulpaep S, De Schutter I, Libin M, Singh M, Malfroot A, Locht C, Mascart F (2017) Age-Stratified T Cell Responses in Children Infected with Mycobacterium tuberculosis. Front Immunol 8:1059. 

5.     Guérin J, Bigot S, Schneider R, Buchanan SK, Jacob-Dubuisson F (2017) Two-Partner Secretion: Combining Efficiency and Simplicity in the Secretion of Large Proteins for Bacteria-Host and Bacteria-Bacteria Interactions. Front Cell Infect Microbiol 7:148. 

6.     Lesne E, Dupré E, Locht C, Antoine R, Jacob-Dubuisson F (2017) Conformational Changes of an Interdomain Linker Mediate Mechanical Signal Transmission in Sensor Kinase BvgS. J Bacteriol 199: e00114-17.

7.     Levillain F, Poquet Y, Mallet L, Mazères S, Marceau M, Brosch R, Bange FC, Supply P, Magalon A, Neyrolles O (2017) Horizontal acquisition of a hypoxia-responsive molybdenum cofactor biosynthesis pathway contributed to Mycobacterium tuberculosis pathoadaptation. PLoS Pathog 13: e1006752. 

8.     Locht C, Papin JF, Lecher S, Debrie AS, Thalen M, Solovay K, Rubin K, Mielcarek N (2017) Live attenuated pertussis vaccine BPZE1 protects baboons against Bordetella pertussis disease and infection. J Infect Dis 216:117-124.

9.     Nikiforov PO, Blaszczyk M, Surade S, Boshoff HI, Sajid A, Delorme V, Deboosere N, Brodin P, Baulard AR, Barry CE 3rd, Blundell TL, Abell C (2017) Fragment-Sized EthR Inhibitors Exhibit Exceptionally Strong Ethionamide Boosting Effect in Whole-Cell Mycobacterium tuberculosis Assays. ACS Chem Biol 12:1390-1396.

10.  Rasigade JP, Barbier M, Dumitrescu O, Pichat C, Carret G, Ronnaux-Baron AS, Blasquez G, Godin-Benhaim C, Boisset S, Carricajo A, Jacomo V, Fredenucci I, Pérouse de Montclos M, Flandrois JP, Ader F, Supply P, Lina G, Wirth T (2017) Strain-specific estimation of epidemic success provides insights into the transmission dynamics of tuberculosis. Sci Rep 7:45326.

11.  Rivera-Millot A, Lesne E, Solans L, Coutte L, Bertrand-Michel J, Froguel P, Dhennin V, Hot D, Locht C, Antoine R, Jacob-Dubuisson F (2017) Characterization of a Bvg-regulated fatty acid methyl-transferase in Bordetella pertussis. PLoS One 12: e0176396.

12.  Salzano G, Wankar J, Ottani S, Villemagne B, Baulard AR, Willand N, Brodin P, Manet I, Gref R (2017) Cyclodextrin-based nanocarriers containing a synergic drug combination: A potential formulation for pulmonary administration of antitubercular drugs. Int J Pharm 531:577-587

13.  Song OR, Deboosere N, Delorme V, Queval CJ, Deloison G, Werkmeister E, Lafont F, Baulard A, Iantomasi R, Brodin P (2017) Phenotypic assays for Mycobacterium tuberculosis infection. Cytometry A 91:983-994

14.  Tagliani E, Hassan MO, Waberi Y, De Filippo MR, Falzon D, Dean A, Zignol M, Supply P, Abdoulkader MA, Hassangue H, Cirillo DM (2017) Culture and Next-generation sequencing-based drug susceptibility testing unveil high levels of drug-resistant-TB in Djibouti: results from the first national survey. Sci Rep 7:17672. 

15.  Tatum NJ, Liebeschuetz JW, Cole JC, Frita R, Herledan A, Baulard AR, Willand N, Pohl E (2017) New active leads for tuberculosis booster drugs by structure-based drug discovery. Org Biomol Chem 15:10245-10255.

16.  Wohlkönig A, Remaut H, Moune M, Tanina A, Meyer F, Desroses M, Steyaert J, Willand N, Baulard AR, Wintjens R (2017) Structural analysis of the interaction between spiroisoxazoline SMARt-420 and the Mycobacterium tuberculosis repressor EthR2. Biochem Biophys Res Commun 487:403-408.




1.   Guérin J, Bigot S, Schneider R, Buchanan SK, Jacob-Dubuisson F (2017) Two-Partner Secretion: Combining Efficiency and Simplicity in the Secretion of Large Proteins for Bacteria-Host and Bacteria-Bacteria Interactions. Front Cell Infect Microbiol 7:148.

2.   Kowalewicz-Kulbat M, Locht C (2017) BCG and protection against inflammatory and auto-immune diseases. Expert Rev Vaccines 16:1-10.




Research Articles


1.      Boritsch EC, Frigui W, Cascioferro A, Malaga W, Etienne G,  Laval F, Pawlik A, Le Chevalier F, Orgeur M, Ma L, Bouchier C, Stinear TP, Supply P, Majlessi L, Daffé M, Guilhot C, Brosch R (2016) pks5-recombination-mediated surface remodelling in Mycobacterium tuberculosis emergence. Nat Microbiol 1: 15019.

2.      Boritsch EC, Khanna V, Pawlik A, Honore N, Navas VH, Ma L, Bouchier C, Seemann T, Supply P, Stinear TP, Brosch R (2016) Key experimental evidence of chromosomal DNA transfer among selected tuberculosis-causing mycobacteria. Proc Natl Acad Sci 113: 9876-81.

3.      Carbonetti NH, Wirsing von Konig CH, Lan R, Jacob-Dubuisson F, Cotter PA, Deora R, Merkel TJ, van Els CA, Locht C, Hozbor D, Rodriguez ME (2016) Highlights of the 11th International Bordetella Symposium: from Basic Biology to Vaccine Development. Clin Vaccine Immunol 23: 842-850.

4.      Coutte L, Huot L, Antoine R, Slupek S, Merkel TJ, Chen Q, Stibitz S, Hot D, Locht C (2016) The multifaceted RisA regulon of Bordetella pertussis. Sci Rep 6: 32774.

5.      Dirix V, Schepers K, Massinga-Loembe M, Worodria W, Colebunders R, Singh M, Locht C, Kestens L, Mascart F (2016) Added Value of Long-Term Cytokine Release Assays to Detect Mycobacterium tuberculosis Infection in HIV-Infected Subjects in Uganda. J Acquir Immune Defic Syndr 72: 344-352.

6.      Feunou PF, Mielcarek N, Locht C (2016) Reciprocal interference of maternal and infant immunization in protection against pertussis. Vaccine 34: 1062-1069.

7.      Lesne E, Krammer EM, Dupre E, Locht C, Lensink MF, Antoine R, Jacob-Dubuisson F (2016) Balance between Coiled-Coil Stability and Dynamics Regulates Activity of BvgS Sensor Kinase in Bordetella. MBio 7: e02089.

8.      Nikiforov PO, Surade S, Blaszczyk M, Delorme V, Brodin P, Baulard AR, Blundell TL, Abell C (2016) A fragment merging approach towards the development of small molecule inhibitors of Mycobacterium tuberculosis EthR for use as ethionamide boosters. Org Biomol Chem 14: 2318-2326.

9.      Pankhurst LJ, Del Ojo Elias C, Votintseva AA, Walker TM, Cole K, Davies J, Fermont JM, Gascoyne-Binzi DM, Kohl TA, Kong C, Lemaitre N, Niemann S, Paul J, Rogers TR, Roycroft E, Smith EG, Supply P, Tang P, Wilcox MH, Wordsworth S, Wyllie D, Xu L, Crook DW; COMPASS-TB Study Group (2016) Rapid, comprehensive, and affordable mycobacterial diagnosis with whole-genome sequencing: a prospective study. Lancet Respir Med 4: 49-58.

10.    Queval CJ, Song OR, Deboosere NDelorme V, Debrie AS, Iantomasi R, Veyron-Churlet R, Jouny S, Redhage K, Deloison G, Baulard A, Chamaillard M, Locht C, Brodin P (2016) STAT3 Represses Nitric Oxide Synthesis in Human Macrophages upon Mycobacterium tuberculosis Infection. Sci Rep 6: 29297.

11.    Rens C, Laval F, Daffe M, Denis O, Frita R, Baulard A, Wattiez R, Lefevre P, Fontaine V (2016) Effects of Lipid-Lowering Drugs on Vancomycin Susceptibility of Mycobacteria. Antimicrob Agents Chemother  60: 6193-6199.

12.    Roisin S, Gaudin C, De Mendonca R, Bellon J, Van Vaerenbergh K, De Bruyne K, Byl B, Pouseele H, Denis O, Supply P (2016) Pan-genome multilocus sequence typing and outbreak-specific reference-based single nucleotide polymorphism analysis to resolve two concurrent Staphylococcus aureus outbreaks in neonatal services. Clin Microbiol Infect 22: 520-526.

13.    Stucki D, Brites D, Jeljeli L, Coscolla M, Q. Liu, (…),Supply P, Comas I, Niemann S, Gagneux S (2016) Mycobacterium tuberculosis lineage 4 comprises globally distributed and geographically restricted sublineages. Nat Genet  48: 1535-1543.

14.    Zimmermann N, Thormann V, Hu B, Kohler AB, Imai-Matsushima A, Locht C, Arnett E, Schlesinger LS, Zoller T, Schürmann M, Kaufmann SH, Wardemann H (2016) Human isotype-dependent inhibitory antibody responses against Mycobacterium tuberculosis. EMBO Mol Med 8: 1325-1339.




1.   Locht C (2016) Tuberculosis, a story still relevant. Med Sci (Paris) 32: 535-536.

2.   Locht C (2016) Pertussis: Where did we go wrong and what can we do about it? J Infect 72 Suppl S34-40.

3.   Locht C (2016) Pertussis: acellular, whole-cell, new vaccines, what to choose? Expert Rev Vaccines 15: 671-3.

4.   Locht C (2016) Live pertussis vaccines: will they protect against carriage and spread of pertussis? Clin Microbiol Infect  22 Suppl 5:S96-S102.

5.   Mielcarek N (2016) La coqueluche, une maladie plus que jamais d'actualité. Feuillets de biologie (ed Orion) 332: 21-28.

6.   Niemann S, Merker M, Kohl T, Supply P (2016)  Impact of Genetic Diversity on the Biology of Mycobacterium tuberculosis Complex Strains. Microbiol Spectr 4.




Research Articles


1.      Béraud G, Kazmercziak S, Beutels P, Levy-Bruhl D, Lenne X, Mielcarek N, Yazdanpanah Y, Boëlle PY, Hens N, Dervaux B (2015) The French Connection: The First Large Population-Based Contact Survey in France Relevant for the Spread of Infectious Diseases. PLoS One 10:e0133203.

2.      Bouklata, N, Supply P, Jaouhari S, Charof R, Seghrouchni F, Sadki K, El Achhab Y, Nejjari C, Filali-Maltouf A, Lahlou O, El Aouad R (2015) Molecular Typing of Mycobacterium Tuberculosis Complex by 24-Locus Based MIRU-VNTR Typing in Conjunction with Spoligotyping to Assess Genetic Diversity of Strains Circulating in Morocco. PLoS One 10: e0135695.

3.      Delorme, V, Song OR, Baulard A, Brodin P (2015) Testing chemical and genetic Modulators in Mycobacterium tuberculosis infected cells using phenotypic assays. Methods Mol Biol 1285: 387-411.

4.      Dupre E, Herrou J, Lensink MF, Wintjens R, Vagin A, Lebedev A, Crosson S, Villeret V, Locht C, Antoine R, Jacob-Dubuisson F (2015) Virulence regulation with Venus flytrap domains: structure and function of the periplasmic moiety of the sensor-kinase BvgS. PLoS Pathog 11: e1004700.

5.      Dupre E, Lesne E, Guerin J, Lensink MF, Verger A, de Ruyck J, Brysbaert G, Vezin H, Locht C, Antoine R, Jacob-Dubuisson F (2015) Signal transduction by BvgS sensor kinase. Binding of modulator nicotinate affects the conformation and dynamics of the entire periplasmic moiety. J Biol Chem 290: 26473.

6.      Fedele G, Sanseverino I, D'Agostino K, Schiavoni I, Locht C, Horenstein AL, Malavasi F, Ausiello C (2015) Unconventional, adenosine-producing suppressor T cells induced by dendritic cells exposed to BPZE1 pertussis vaccine. J Leukoc Biol 98: 631-639.

7.      Guerin J, Saint N, Baud C, Meli AC, Etienne E, Locht C, Vezin H, Jacob-Dubuisson F (2015) Dynamic interplay of membrane-proximal POTRA domain and conserved loop L6 in Omp85 transporter FhaC.  Mol Microbiol 98: 490-501.

8.      Maier T, Clantin B, Gruss F, Dewitte F, Delattre AS, Jacob-Dubuisson F, Hiller S, Villeret V (2015) Conserved Omp85 lid-lock structure and substrate recognition in FhaC. Nat Commun 6: 7452.

9.      Merker M, Blin C, Mona S, Duforet-Frebourg N, Lecher S,  Willery E, (…), Supply P*, Niemann S, Wirth T (2015) Evolutionary history and global spread of the Mycobacterium tuberculosis Beijing lineage. Nat Genet 47: 242-249. *Co-senior author

10.    Mori G, Chiarelli LR, Esposito M, (…), Baulard AR, Mikušová K, Alzari PM, Manganelli R, de Carvalho LP, Riccardi G, Cole ST, Pasca MR (2015) Thiophenecarboxamide Derivatives Activated by EthA Kill Mycobacterium tuberculosis by Inhibiting the CTP Synthetase PyrG. Chem Biol 22: 917-927.

11.    Papadimitriou K, Anastasiou R, Maistrou E, Plakas T, Papandreou NC, Hamodrakas SJ, Ferreira S, Supply P, Renault P, Pot B, Tsakalidou E (2015) Acquisition through horizontal gene transfer of plasmid pSMA198 by Streptococcus macedonicus ACA-DC 198 points towards the dairy origin of the species. PLoS One 10: e0116337.

12.    Schepers K, Dirix V, Mouchet F, Verscheure V, Lecher S, Locht C, Mascart F (2015) Early cellular immune response to a new candidate mycobacterial vaccine antigen in childhood tuberculosis. Vaccine 33: 1077-1083.

13.    Szpakowski P, Biet F, Locht C, Paszkiewicz M, Rudnicka W, Druszczynska M, Allain F, Fol M, Pestel J, Kowalewicz-Kulbat M (2015) Dendritic Cell Activity Driven by Recombinant Mycobacterium bovis BCG Producing Human IL-18, in Healthy BCG Vaccinated Adults. J Immunol Res 2015: 359153.

14.    Walker TM, Kohl TA, Omar SV, Hedge J, Del Ojo Elias C, Bradley P, Iqbal Z, Feuerriegel S, Niehaus KE, Wilson DJ, Clifton DA, Kapatai G, Ip CLC, Bowden R, Drobniewski FA, Allix-Béguec C, Gaudin C, Parkhill J, Diel R, Supply P, Crook DW, Smith EG, Walker AS, Ismail N, Niemann S, Peto TEA; Modernizing Medical Microbiology (MMM) Informatics Group (2015) Whole-genome sequencing for prediction of Mycobacterium tuberculosis drug susceptibility and resistance: a retrospective cohort study. Lancet Infect Dis 15: 1193-1202.

15.    Wyndham-Thomas C, Dirix V, Schepers K, Martin C, Hildebrand M, Goffard JC, Domont F, Libin M, Loyens M, Locht C, Van Vooren JP, Mascart F (2015) Contribution of a heparin-binding haemagglutinin interferon-gamma release assay to the detection of Mycobacterium tuberculosis infection in HIV-infected patients: comparison with the tuberculin skin test and the QuantiFERON-TB Gold In-tube. BMC Infect Dis 15: 59.




1.   Coutte L, Locht C (2015) Investigating pertussis toxin and its impact on vaccination. Future Microbiol 10: 241-254.

2.   Jacob-Dubuisson F, Holland IB (2015) Editorial overview: Elucidation of protein translocation pathways, part I. J Mol Biol 427: 997-998.

3.   Mascart F, Locht C (2015) Integrating knowledge of Mycobacterium tuberculosis pathogenesis for the design of better vaccines. Expert Rev Vaccines 14: 1573-1585.




Research Articles


1.      Allix-Béguec C, Wahl C, Hanekom M, Nikolayevskyy V, Drobniewski F, Maeda S, Campos-Herrero I, Mokrousov I, Niemann S, Kontsevaya I, Rastogi N, Samper S, Sng LH, Warren RM, Supply P (2014) Proposal of a consensus set of hypervariable mycobacterial interspersed repetitive-unit-variable-number tandem-repeat loci for subtyping of Mycobacterium tuberculosis Beijing isolates. J Clin Microbiol 52: 164-172.

2.      Alvarez, A.S., L. Remy, C. Allix-Beguec, C. Ligier, C. Dupont, Leminor O, Lawrence C, Supply P, Guillemot D, Gaillard JL, Salomon J, Herrmann JL (2014) Patient nostril microbial flora: individual-dependency and diversity precluding prediction of Staphylococcus aureus acquisition. Clin Microbiol Infect 20: 70-78.

3.      Baud C, Guérin J, Petit E, Lesne E, Dupré E, Locht C, Jacob-Dubuisson F (2014) Translocation path of a substrate protein through its Omp85 transporter. Nat Commun 5: 5271.

4.      Bidovec-Stojkovič U, Seme K, Žolnir-Dovč M, Supply P (2014) Prospective genotyping of Mycobacterium tuberculosis from fresh clinical samples. PLoS One 9: e109547.

5.      Boritsch EC, Supply P, Honoré N, Seemann T, Stinear TP, Brosch R (2014) A glimpse into the past and predictions for the future: the molecular evolution of the tuberculosis agent. Mol Microbiol 93: 835-852.

6.      Bottai D, Stinear TP, Supply P, Brosch R (2014) Mycobacterial Pathogenomics and Evolution. Microbiol Spectr 2: MGM2-0025-2013.

7.      Coitinho C, Greif G, Robello C, Laserra P, Willery E, Supply P (2014) Rapidly progressing tuberculosis outbreak in a very low risk group. Eur Respir J 43: 903-906.

8.      Crauste C, Willand N, Villemagne B, Flipo M, Willery E, Carette X, Dimala MM, Drucbert AS, Danze PM, Deprez B, Baulard AR (2014) Unconventional surface plasmon resonance signals reveal quantitative inhibition of transcriptional repressor EthR by synthetic ligands. Anal Biochem 452: 54-66.

9.      de Beer JL, Ködmön C, van Ingen J, Supply P, van Soolingen D (2014) Second worldwide proficiency study on variable number of tandem repeats typing of Mycobacterium tuberculosis complex. Int J Tuberc Lung Dis, 2014. 18(5): p. 594-600.

10.    Dirix V, Mielcarek N, Debrie AS, Willery E, Alonso S, Versheure V, Mascart F, Locht C (2014) Human dendritic cell maturation and cytokine secretion upon stimulation with Bordetella pertussis filamentous haemagglutinin. Microbes Infect 16: 562-570.

11.    Feunou PF, Kammoun H, Debrie AS, Locht C (2014) Heterologous prime-boost immunization with live attenuated Bordetella pertussis BPZE1 followed by acellular pertussis vaccine in mice. Vaccine 32: 4281-4288.

12.    Gabel F, Lensink MF, Clantin B, Jacob-Dubuisson F, Villeret V, Ebel C (2014) Probing the conformation of FhaC with small-angle neutron scattering and molecular modeling. Biophys J 107: 185-196.

13.    Guérin JBaud C, Touati N, Saint N, Willery E, Locht C, Vezin H, Jacob-Dubuisson F (2014) Conformational dynamics of protein transporter FhaC: large-scale motions of plug helix. Mol Microbiol 92: 1164-1176.

14.    Jahnmatz M, Amu S, Ljungman M, Wehlin L, Chiodi F, Mielcarek N, Locht C, Thorstensson R (2014) B-cell responses after intranasal vaccination with the novel attenuated Bordetella pertussis vaccine strain BPZE1 in a randomized phase I clinical trial. Vaccine 32: 3350-3356.

15.    Leiba J, Carrère-Kremer S, Blondiaux N, Dimala MM, Wohlkönig A, Baulard A, Kremer L, Molle V (2014) The Mycobacterium tuberculosis transcriptional repressor EthR is negatively regulated by Serine/Threonine phosphorylation. Biochem Biophys Res Commun 446: 1132-1138.

16.    Lim A, Ng JK, Locht C, Alonso S (2014) Protective role of adenylate cyclase in the context of a live pertussis vaccine candidate. Microbes Infect 16: 51-60.

17.    Norell D, Heuck A, Tran-Thi TA, Götzke H, Jacob-Dubuisson F, Clausen T, Daley DO, Braun V, Müller M, Fan E (2014) Versatile in vitro system to study translocation and functional integration of bacterial outer membrane proteins. Nat Commun 5: 5396.

18.    Papadimitriou K, Anastasiou R, Mavrogonatou E, Blom J, Papandreou NC, Hamodrakas SJ, Ferreira S, Renault P, Supply P, Pot B, Tsakalidou E (2014) Comparative genomics of the dairy isolate Streptococcus macedonicus ACA-DC 198 against related members of the Streptococcus bovis/Streptococcus equinus complex. BMC Genomics 15: 272.

19.    Queval CJ, Song OR, Delorme V, Iantomasi R, Veyron-Churlet R, Deboosère N, Landry V, Baulard A, Brodin P (2014) A microscopic phenotypic assay for the quantification of intracellular mycobacteria adapted for high-throughput/high-content screening. J Vis Exp 83: e51114.

20.    Salcedo-Rivillas C, Debrie AS, Miyaji EN, Ferreira JM Jr, Raw I, Locht C, Ho PL, Mielcarek N, Oliveira ML (2014) Pertussis toxin improves immune responses to a combined pneumococcal antigen and leads to enhanced protection against Streptococcus pneumoniae. Clin Vaccine Immunol 21: 972-981.

21.    Schiavoni I, Fedele G, Quattrini A, Bianco M, Schnoeller C, Openshaw PJ, Locht C, Ausiello CM (2014) Live attenuated Bordetella pertussis BPZE1 rescues the immune functions of Respiratory Syncytial virus infected human dendritic cells by promoting Th1/Th17 responses. PLoS One 9: e100166.

22.    Schnoeller C, Roux X, Sawant D, Raze D, Olszewska W, Locht C, Openshaw PJ (2014) Attenuated Bordetella pertussis vaccine protects against respiratory syncytial virus disease via an IL-17-dependent mechanism. Am J Respir Crit Care Med 189: 194-202.

23.    Stylianou E, Diogo GR, Pepponi I, van Dolleweerd C, Arias MA, Locht C, Rider CC, Sibley L, Cutting SM, Loxley A, Ma JK, Reljic R (2014) Mucosal delivery of antigen-coated nanoparticles to lungs confers protective immunity against tuberculosis infection in mice. Eur J Immunol 44: 440-9.

24.    Supply P, Gaudin C, Raze D (2014) Optimization of standard 24-locus variable-number tandem-repeat typing of Mycobacterium tuberculosis isolates: a multicenter perspective. J Clin Microbiol 52: 3518-3519.

25.    Thorstensson R, Trollfors B, Al-Tawil N, Jahnmatz M, Bergström J, Ljungman M, Törner A, Wehlin L, Van Broekhoven A, Bosman F, Debrie AS, Mielcarek N, Locht C (2014) A phase I clinical study of a live attenuated Bordetella pertussis vaccine--BPZE1; a single centre, double-blind, placebo-controlled, dose-escalating study of BPZE1 given intranasally to healthy adult male volunteers. PLoS One 9: e83449.

26.    Toit K, Altraja A, Acosta CD, Viiklepp P, Kremer K, Kummik T, Danilovitš M, Van den Bergh R, Harries AD, Supply P (2014) A four-year nationwide molecular epidemiological study in Estonia: risk factors for tuberculosis transmission. Public Health Action 4(Suppl 2): S34-40.

27.    Verwaerde C, Debrie AS, Dombu C, Legrand D, Raze D, Lecher S, Betbeder D, Locht C (2014) HBHA vaccination may require both Th1 and Th17 immune responses to protect mice against tuberculosis. Vaccine 32: 6240-6250.

28.    Villemagne B, Flipo M, Blondiaux N, Crauste C, Malaquin S, Leroux F, Piveteau C, Villeret V, Brodin P, Villoutreix BO, Sperandio O, Soror SH, Wohlkönig A, Wintjens R, Deprez B, Baulard AR, Willand N (2014) Ligand efficiency driven design of new inhibitors of Mycobacterium tuberculosis transcriptional repressor EthR using fragment growing, merging, and linking approaches. J Med Chem 57: 4876-4888.

29.    Wyndham-Thomas C, Corbière V, Dirix V, Smits K, Domont F, Libin M, Loyens M, Locht C, Mascart F (2014) Key role of effector memory CD4+ T lymphocytes in a short-incubation heparin-binding hemagglutinin gamma interferon release assay for the detection of latent tuberculosis. Clin Vaccine Immunol 21: 321-328.




1.   Locht C, Mielcarek N (2014) Live attenuated vaccines against pertussis. Expert Rev Vaccines 13: 1147-1158.

2.   Meade BD, Plotkin SA, Locht C (2014) Possible options for new pertussis vaccines. J Infect Dis 209 Suppl 1: S24-27.

3.   Niemann S, Supply P (2014) Diversity and evolution of Mycobacterium tuberculosis: moving to whole-genome-based approaches. Cold Spring Harb Perspect Med 4: a021188.

4.   van Els CA, Corbiere V, Smits K, van Gaans-van den Brink JA, Poelen MC, Mascart F, Meiring HD, Locht C (2014) Toward Understanding the Essence of Post-Translational Modifications for the Mycobacterium tuberculosis Immunoproteome. Front Immunol 5: 361.




Research Articles


1.      Alsteens D, Martinez N, Jamin M, Jacob-Dubuisson F (2013) Sequential unfolding of beta helical protein by single-molecule atomic force microscopy. PLoS One 8: e73572.

2.      de Beer JL, van Ingen J, de Vries G, Erkens C, Sebek M, Mulder A, Sloot R, van den Brandt AM, Enaimi M, Kremer K, Supply P, van Soolingen D (2013) Comparative study of IS6110 restriction fragment length polymorphism and variable-number tandem-repeat typing of Mycobacterium tuberculosis isolates in the Netherlands, based on a 5-year nationwide survey. J Clin Microbiol 51: 1193-8.

3.      Dessein, R., V. Corbiere, J. Nortier, M. Dratwa, K. Gastaldello, A. Pozdzik, S. Lecher, B. Grandbastien, C. Locht, and F. Mascart (2013) Heparin-binding haemagglutinin, a new tool for the detection of latent Mycobacterium tuberculosis infection in hemodialysis patients. PLoS One 8:  e71088.

4.      Dupre E, Wohlkonig A, Herrou J, Locht C, Jacob-Dubuisson F, Antoine R (2013) Characterization of the PAS domain in the sensor-kinase BvgS: mechanical role in signal transmission. BMC Microbiol 13: 172.

5.      Fontaine L, Goffin P, Dubout H, Delplace B, Baulard A, Lecat-Guillet N, Chambellon E, Gardan R, Hols P (2013) Mechanism of competence activation by the ComRS signalling system in streptococci. Mol Microbiol 87: 1113-1132.

6.      Kammoun H, Roux X, Raze D, Debrie AS, De Filette M, Ysenbaert T, Mielcarek N, Saelens X, Fiers W, Locht C (2013) Immunogenicity of live attenuated Bordetella pertussis BPZE1 producing the universal influenza vaccine candidate M2e. PLoS One 8: e59198.

7.      Lefrancois LH, Bodier CC, Cochard T, Canepa S, Raze D, Lanotte P, Sevilla IA, Stevenson K, Behr MA, Locht C, Biet F (2013) Novel feature of Mycobacterium avium subsp. paratuberculosis, highlighted by characterization of the heparin-binding hemagglutinin adhesin. J Bacteriol 195: 4844-4853.

8.      Lefrancois LH, Bodier CC, Lecher S, Gilbert FB, Cochard T, Harichaux G, Labas V, Teixeira-Gomes AP, Raze D, Locht C, Biet F (2013) Purification of native HBHA from Mycobacterium avium subsp. paratuberculosis. BMC Res Notes 6: 55.

9.      Roetzer A, Diel R, Kohl TA, Ruckert C, Nubel U, Blom J, Wirth T, Jaenicke S, Schuback S, Rüsch-Gerdes S, Supply P, Kalinowski J, Niemann S (2013) Whole genome sequencing versus traditional genotyping for investigation of a Mycobacterium tuberculosis outbreak: a longitudinal molecular epidemiological study. PLoS Med 10: e1001387.

10.    Sloot R, Borgdorff MW, de Beer JL, van Ingen J, Supply P, van Soolingen D (2013) Clustering of tuberculosis cases based on variable-number tandem-repeat typing in relation to the population structure of Mycobacterium tuberculosis in the Netherlands. J Clin Microbiol 51: 2427-2431.

11.    Smits K, Pottier G, Smet J, Dirix V, Vermeulen F, De Schutter I, Carollo M, Locht C, Ausiello CM, Mascart F (2013) Different T cell memory in preadolescents after whole-cell or acellular pertussis vaccination. Vaccine 32: 111-118.

12.    Stoffels K, Allix-Beguec C, Groenen G, Wanlin M, Berkvens D, Mathys V, Supply P, Fauville-Dufaux M (2013) From multidrug- to extensively drug-resistant tuberculosis: upward trends as seen from a 15-year nationwide study. PLoS One 8: e63128.

13.    Supply P, Marceau M, Mangenot S, Roche D, Rouanet C, Khanna V, Majlessi L, Criscuolo A, Tap J, Pawlik A, Fiette L, Orgeur M, Fabre M, Parmentier C, Frigui W, Simeone R, Boritsch EC, Debrie AS, Willery E, Walker D, Quail MA, Ma L, Bouchier C, Salvignol G, Sayes F, Cascioferro A, Seemann T, Barbe V, Locht C, Gutierrez MC, Leclerc C, Bentley SD, Stinear TP, Brisse S, Médigue C, Parkhill J, Cruveiller S, Brosch R (2013) Genomic analysis of smooth tubercle bacilli provides insights into ancestry and pathoadaptation of Mycobacterium tuberculosis. Nat Genet 45: 172-179.

14.    Tatum NJ, Villemagne B, Willand N, Deprez B, Liebeschuetz JW, Baulard AR, Pohl E (2013) Structural and docking studies of potent ethionamide boosters. Acta Crystallogr C 69: 1243-1250.

15.    Varghese BSupply P, Allix-Béguec C, Shoukri M, Al-Omari R, Herbawi M, Al-Hajoj S (2013) Admixed phylogenetic distribution of drug resistant Mycobacterium tuberculosis in Saudi Arabia. PLoS One 8: e55598.

16.    Varghese BSupply P, Shoukri M, Allix-Beguec C, Memish Z, Abuljadayel N, Al-Hakeem R, AlRabiah F, Al-Hajoj S (2013) Tuberculosis transmission among immigrants and autochthonous populations of the eastern province of Saudi Arabia. PLoS One 8: e77635.

17.    Vermeulen F, Dirix V, Verscheure V, Damis E, Vermeylen D, Locht C, Mascart F (2013) Persistence at one year of age of antigen-induced cellular immune responses in preterm infants vaccinated against whooping cough: comparison of three different vaccines and effect of a booster dose. Vaccine 31: 1981-1986.




1.    Jacob-Dubuisson F, Guerin J, Baelen S, Clantin B (2013) Two-partner secretion: as simple as it sounds? Res Microbiol 164: 583-595.